TCR V beta 11 Monoclonal Antibody (RR3-15), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: RR3-15
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR. In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule. Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity.
The RR3-15 antibody has been reported to activate V beta 11+ T cells.
Applications Reported: The RR3-15 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RR3-15 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: RR3-15
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR. In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule. Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity.
The RR3-15 antibody has been reported to activate V beta 11+ T cells.
Applications Reported: The RR3-15 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RR3-15 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: RR3-15
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR. In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule. Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity.
The RR3-15 antibody has been reported to activate V beta 11+ T cells.
Applications Reported: The RR3-15 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RR3-15 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
