MULT1 (NKG2D Ligand) Monoclonal Antibody (5D10), eBioscience
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: 5D10
Reactivity: Ms, Rt
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 5D10 monoclonal antibody reacts with the murine MULT-1 (Murine ULBP-Like Transcript 1). MULT-1 is a type I transmembrane protein with two Ig-like domain and is one of the known murine NKG2-D ligands that include RAE1 molecules (alpha, beta, epsilon, gamma and delta) and H60. MULT-1 is a high affinity ligand for NKG2D.Expression of NKG2-D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2-D ligand and can activate NK cell tumoricidal activity. Until now, the expression of NKG2-D ligands has been mainly studied with NKG2-D tetramers recognizing all NKG2-D ligands.
Applications Reported: This 5D10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This 5D10 antibody has been tested by flow cytometric analysis of the A20 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: 5D10
Reactivity: Ms, Rt
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 5D10 monoclonal antibody reacts with the murine MULT-1 (Murine ULBP-Like Transcript 1). MULT-1 is a type I transmembrane protein with two Ig-like domain and is one of the known murine NKG2-D ligands that include RAE1 molecules (alpha, beta, epsilon, gamma and delta) and H60. MULT-1 is a high affinity ligand for NKG2D.Expression of NKG2-D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2-D ligand and can activate NK cell tumoricidal activity. Until now, the expression of NKG2-D ligands has been mainly studied with NKG2-D tetramers recognizing all NKG2-D ligands.
Applications Reported: This 5D10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This 5D10 antibody has been tested by flow cytometric analysis of the A20 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: 5D10
Reactivity: Ms, Rt
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 5D10 monoclonal antibody reacts with the murine MULT-1 (Murine ULBP-Like Transcript 1). MULT-1 is a type I transmembrane protein with two Ig-like domain and is one of the known murine NKG2-D ligands that include RAE1 molecules (alpha, beta, epsilon, gamma and delta) and H60. MULT-1 is a high affinity ligand for NKG2D.Expression of NKG2-D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2-D ligand and can activate NK cell tumoricidal activity. Until now, the expression of NKG2-D ligands has been mainly studied with NKG2-D tetramers recognizing all NKG2-D ligands.
Applications Reported: This 5D10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This 5D10 antibody has been tested by flow cytometric analysis of the A20 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
