PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: Ab204

Format: Alexa Fluor 488

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 5 µl (0.5 µg)/test

Concentration: 5 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This Ab204 monoclonal antibody detects human DDX5 protein also known as DEAD box protein 5 and RNA helicase p68. This antibody has been reported to cross-react with mouse and rat orthologs of DDX5 protein.

Applications Reported: This Ab204 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Ab204 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Applications Reported: This Ab204 antibody has been reported for use in immunocytochemical staining.

Applications Tested: This Ab204 antibody has been pre-diluted and tested by immunocytochemical staining on cultured A549 cells using PFA fixation. This may be used at 2.5 µL (0.025 µg) per ml.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.