PRODUCT DETAILS

Host: Mouse

Isotype: IgG2a

Clonality: Monoclonal

Clone: 2B5

Format: FITC

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 0.1 mg/mL

Storage: 4°C, store in dark

Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4

Purification: Protein G

Data Sheet: TDS

Specific Information

Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.

Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.

Applications Tested: Flow Cytometry.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.