PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: BBC3M4

Format: PE

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 5 µL (0.125 µg)/test

Concentration: 5 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This BBC3M4 monoclonal antibody reacts with human CD199 (CCR9), a 7 transmembrane spanning G-protein-coupled receptor. CCR9 expression is regulated during thymic development and CCR9 deficiency results in mild impairment in thymocyte development. In the periphery, CCR9 and alpha 4 beta 7 integrin are upregulated in response to dendritic cell-derived retinoic acid, conferring preferential trafficking to gut-associated tissues. CCR9 is the receptor for CCL25 (TECK).

Applications Reported: This BBC3M4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This BBC3M4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.